safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
JEKAMKIP_1	728342	729211	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
JEKAMKIP_1	729211	729804	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
JEKAMKIP_1	517891	518700	STU_RS16245	Capsule	Immune modulation	99.88	100.00	WP_002948020
JEKAMKIP_1	729211	729804	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
JEKAMKIP_1	516951	517907	STU_RS16250	Capsule	Immune modulation	99.79	100.00	WP_011226328
JEKAMKIP_1	515806	516954	STU_RS16255	Capsule	Immune modulation	99.74	100.00	WP_041828290
JEKAMKIP_1	518700	519905	STU_RS16240	Capsule	Immune modulation	99.67	100.00	WP_011226326
JEKAMKIP_1	729211	729804	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
JEKAMKIP_1	511459	512310	rfbD	Capsule	Immune modulation	99.65	100.00	WP_014621820
JEKAMKIP_1	968235	969893	fbp54	FBPs	Adherence	99.58	100.00	WP_011227179
JEKAMKIP_1	520977	522761	STU_RS16230	Capsule	Immune modulation	99.50	100.00	WP_011226325
JEKAMKIP_1	514687	515697	STU_RS16260	Capsule	Immune modulation	99.50	100.00	WP_011226330
JEKAMKIP_1	519925	520980	STU_RS16235	Capsule	Immune modulation	99.43	100.00	WP_002948022
JEKAMKIP_1	43684	44523	cbpD	Autolysin	Exoenzyme	99.41	100.00	WP_011226733
JEKAMKIP_1	692259	693020	srtA	Sortase A	Exoenzyme	99.21	100.00	-
JEKAMKIP_1	855735	856466	STER_RS05315	Capsule	Immune modulation	99.04	100.00	WP_011681176
JEKAMKIP_1	854274	855734	STER_RS05320	Capsule	Immune modulation	98.97	100.00	WP_011681177
JEKAMKIP_1	860529	861944	STER_RS05250	Capsule	Immune modulation	98.31	100.00	WP_011681165
JEKAMKIP_1	855735	856466	STR_RS05260	Capsule	Immune modulation	98.09	100.00	WP_011227248
JEKAMKIP_1	786613	787408	cshA	Cell surface hydrophobicity proteins	Adherence	97.99	100.51	-
JEKAMKIP_1	855735	856466	STU_RS14645	Capsule	Immune modulation	97.95	100.00	WP_011226048
JEKAMKIP_1	513413	514684	STU_RS16265	Capsule	Immune modulation	97.48	99.76	WP_011226331
JEKAMKIP_1	854274	855734	STU_RS14650	Capsule	Immune modulation	97.26	100.21	WP_011226049
JEKAMKIP_1	857177	857918	STU_RS14635	Capsule	Immune modulation	97.17	100.00	WP_011226046
JEKAMKIP_1	857177	857918	STER_RS05305	Capsule	Immune modulation	96.77	100.00	WP_011681174
JEKAMKIP_1	854274	855734	STR_RS05265	Capsule	Immune modulation	96.72	100.21	WP_041827043
JEKAMKIP_1	857177	857913	STR_RS05250	Capsule	Immune modulation	96.20	98.13	WP_011227246
JEKAMKIP_1	857975	859342	STU_RS14630	Capsule	Immune modulation	95.61	100.00	WP_011226045
JEKAMKIP_1	856475	857167	STR_RS05255	Capsule	Immune modulation	95.25	99.86	WP_011227247
JEKAMKIP_1	856475	857167	STU_RS14640	Capsule	Immune modulation	95.25	99.86	WP_011226047
JEKAMKIP_1	857975	859340	STER_RS05300	Capsule	Immune modulation	94.95	99.85	WP_011681173
JEKAMKIP_1	856475	857167	STER_RS05310	Capsule	Immune modulation	89.75	100.00	WP_011681175
JEKAMKIP_1	1214628	1215931	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
JEKAMKIP_1	1607709	1608719	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	87.55	99.90	WP_002986042
JEKAMKIP_1	161680	162963	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
JEKAMKIP_1	730233	731279	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
JEKAMKIP_1	689572	688589	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
JEKAMKIP_1	1111053	1109980	MntE	0.0	manganese homeostasis	94.13	99.72	ABF32167
JEKAMKIP_1	1298213	1297245	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
JEKAMKIP_1	1484640	1486943	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
JEKAMKIP_1	621293	619812	SPD_0310	0.0	UPF0371 protein SPD_0310	92.31	100.00	ABJ54480
JEKAMKIP_1	1089141	1086895	metE	0.0	methionine biosynthesis enzymes	91.86	100.00	AAK74738
JEKAMKIP_1	63315	64307	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
JEKAMKIP_1	27201	28172	prsA2	0.0	ribose-phosphate pyrophosphokinase	89.81	100.00	ABF36968
JEKAMKIP_1	460734	461885	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
JEKAMKIP_1	496489	498477	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
JEKAMKIP_1	705769	707499	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
JEKAMKIP_1	1808369	1806891	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
JEKAMKIP_1	1215931	1214630	Eno	0.0	enolase	86.21	99.77	ABP90468
JEKAMKIP_1	1791760	1789868	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
JEKAMKIP_1	1083970	1085679	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
JEKAMKIP_1	1650844	1651854	pdh	0.0	pyruvate dehydrogenase	82.49	99.70	ABP91006
JEKAMKIP_1	940339	941700	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
JEKAMKIP_1	1702864	1700972	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
JEKAMKIP_1	1230367	1229477	bglB	5.16e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
JEKAMKIP_1	1024670	1025605	SP_0676	7.25e-168	transcription facot	81.73	96.89	AAK74821
JEKAMKIP_1	1086703	1085846	metF	1.50e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
JEKAMKIP_1	817448	818152	covR	4.30e-131	responder protein	85.53	100.00	AAK71319
JEKAMKIP_1	640270	640941	rr01	2.95e-124	response regulator	83.93	99.56	CAB54566
JEKAMKIP_1	817448	818152	walR	1.81e-121	DNA-binding response regulator	82.13	100.00	ANC99504
JEKAMKIP_1	316108	316794	CovR	1.01e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
JEKAMKIP_1	1080016	1079348	CiaR	1.98e-117	two-component response regulator	84.31	99.55	AAK74935
JEKAMKIP_1	1080016	1079345	ciaR	1.95e-115	two component system response regulator	82.14	99.12	AIK75708
JEKAMKIP_1	64604	64999	SpxA2	3.61e-73	transcriptional regulator	90.15	100.00	ERL21461
JEKAMKIP_1	928989	928588	SpxA1	3.69e-66	transcriptional regulator	80.60	100.00	ERL19273
JEKAMKIP_1	163121	163621	RopE (M5005_Spy_1611)	4.83e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
JEKAMKIP_1	1484640	1486937	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
JEKAMKIP_1	1688392	1687373	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
JEKAMKIP_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found