safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	29	44.69

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
JBELIJGC_1	43663	44502	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
JBELIJGC_1	1321992	1322843	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
JBELIJGC_1	979562	980485	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
JBELIJGC_1	980445	981395	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
JBELIJGC_1	981420	982502	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
JBELIJGC_1	982592	983566	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
JBELIJGC_1	983572	984051	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
JBELIJGC_1	984244	985362	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
JBELIJGC_1	987053	987652	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
JBELIJGC_1	987789	988538	STR_RS05250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227246
JBELIJGC_1	989249	989980	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
JBELIJGC_1	1101939	1102532	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
JBELIJGC_1	1309513	1311258	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
JBELIJGC_1	1311260	1312960	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
JBELIJGC_1	1314180	1314986	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
JBELIJGC_1	1314986	1315927	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
JBELIJGC_1	1315924	1317072	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
JBELIJGC_1	870574	872232	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
JBELIJGC_1	978133	979554	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
JBELIJGC_1	989981	991435	STR_RS05265	0.0	Capsule	Immune modulation	99.93	100.00	WP_041827043
JBELIJGC_1	1312978	1314180	STR_RS06895	0.0	Capsule	Immune modulation	99.92	100.00	WP_011227428
JBELIJGC_1	975381	976439	waaB	0.0	Capsule	Immune modulation	99.91	100.00	WP_157822584
JBELIJGC_1	985930	986889	STR_RS05240	0.0	Capsule	Immune modulation	99.90	100.00	WP_041827042
JBELIJGC_1	988548	989240	STR_RS05255	0.0	Capsule	Immune modulation	99.86	100.00	WP_011227247
JBELIJGC_1	1101939	1102532	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
JBELIJGC_1	1101939	1102532	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
JBELIJGC_1	1137296	1138055	srtA	0.0	Sortase A	Exoenzyme	99.74	99.87	-
JBELIJGC_1	1311260	1312960	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
JBELIJGC_1	988548	989240	STU_RS14640	0.0	Capsule	Immune modulation	99.57	100.00	WP_011226047
JBELIJGC_1	1312978	1314180	STER_RS07055	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681436
JBELIJGC_1	1314180	1314986	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
JBELIJGC_1	1314986	1315927	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
JBELIJGC_1	1055883	1056673	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
JBELIJGC_1	1315924	1317072	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
JBELIJGC_1	1102532	1103401	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
JBELIJGC_1	989249	989980	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
JBELIJGC_1	987803	988538	STER_RS05305	0.0	Capsule	Immune modulation	98.50	99.33	WP_011681174
JBELIJGC_1	989249	989980	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
JBELIJGC_1	1309513	1311258	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
JBELIJGC_1	989981	991435	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
JBELIJGC_1	987803	988538	STU_RS14635	0.0	Capsule	Immune modulation	97.96	99.33	WP_011226046
JBELIJGC_1	1320611	1321870	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
JBELIJGC_1	989981	991435	STER_RS05320	0.0	Capsule	Immune modulation	96.99	99.79	WP_011681177
JBELIJGC_1	988548	989240	STER_RS05310	0.0	Capsule	Immune modulation	89.75	100.00	WP_011681175
JBELIJGC_1	603262	604565	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
JBELIJGC_1	1603724	1604734	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
JBELIJGC_1	1314180	1314986	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
JBELIJGC_1	149038	150321	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
JBELIJGC_1	1100464	1101510	rfbB	0.0	Capsule	Immune modulation	80.52	100.00	WP_009659423
JBELIJGC_1	1319916	1320614	STER_RS07095	2.94e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
JBELIJGC_1	985638	985874	STR_RS05235*	6.64e-119	Capsule	Immune modulation	100.00	100.00	-
JBELIJGC_1	1319096	1319562	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
JBELIJGC_1	1313443	1314180	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
JBELIJGC_1	1140742	1141725	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
JBELIJGC_1	716635	717708	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
JBELIJGC_1	520989	521957	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
JBELIJGC_1	1484203	1486506	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
JBELIJGC_1	745087	747333	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
JBELIJGC_1	1223226	1224707	SPD_0310	0.0	UPF0371 protein SPD_0310	91.90	100.00	ABJ54480
JBELIJGC_1	63294	64286	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
JBELIJGC_1	27181	28152	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
JBELIJGC_1	1370346	1369195	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
JBELIJGC_1	1335873	1333891	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
JBELIJGC_1	1124550	1122820	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.69	100.00	AAK34199
JBELIJGC_1	1793292	1791814	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
JBELIJGC_1	603262	604563	Eno	0.0	enolase	86.21	99.77	ABP90468
JBELIJGC_1	1778556	1776664	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
JBELIJGC_1	750258	748549	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
JBELIJGC_1	1645418	1646428	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
JBELIJGC_1	897654	896293	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
JBELIJGC_1	1695749	1693857	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
JBELIJGC_1	588832	589722	bglB	2.84e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
JBELIJGC_1	809586	808651	SP_0676	7.19e-168	transcription facot	81.73	96.89	AAK74821
JBELIJGC_1	747525	748382	metF	8.42e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
JBELIJGC_1	1028135	1027431	covR	3.41e-130	responder protein	84.68	100.00	AAK71319
JBELIJGC_1	1193925	1193254	rr01	2.87e-125	response regulator	84.38	99.56	CAB54566
JBELIJGC_1	1028135	1027431	walR	2.45e-120	DNA-binding response regulator	80.85	100.00	ANC99504
JBELIJGC_1	307978	308664	CovR	9.80e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
JBELIJGC_1	754211	754861	CiaR	1.35e-116	two-component response regulator	86.17	96.88	AAK74935
JBELIJGC_1	754211	754861	ciaR	6.94e-114	two component system response regulator	83.87	96.02	AIK75708
JBELIJGC_1	64583	64978	SpxA2	3.61e-74	transcriptional regulator	90.91	100.00	ERL21461
JBELIJGC_1	909013	909414	SpxA1	3.66e-66	transcriptional regulator	80.60	100.00	ERL19273
JBELIJGC_1	150479	150979	RopE (M5005_Spy_1611)	9.62e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
JBELIJGC_1	1484203	1486500	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
JBELIJGC_1	1681288	1680269	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
JBELIJGC_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found