safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	27	28	38.90

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
CKHBGFBL_1	43606	44445	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
CKHBGFBL_1	1306483	1307334	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
CKHBGFBL_1	1300093	1300902	STU_RS16245	0.0	Capsule	Immune modulation	100.00	100.00	WP_002948020
CKHBGFBL_1	1303096	1304106	STU_RS16260	0.0	Capsule	Immune modulation	99.90	100.00	WP_011226330
CKHBGFBL_1	1106016	1106609	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
CKHBGFBL_1	1300886	1301842	STU_RS16250	0.0	Capsule	Immune modulation	99.79	100.00	WP_011226328
CKHBGFBL_1	1296032	1297816	STU_RS16230	0.0	Capsule	Immune modulation	99.72	100.00	WP_011226325
CKHBGFBL_1	1106016	1106609	STER_RS06030	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
CKHBGFBL_1	1106016	1106609	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
CKHBGFBL_1	865600	867258	fbp54	0.0	FBPs	Adherence	99.58	100.00	WP_011227179
CKHBGFBL_1	1298888	1300093	STU_RS16240	0.0	Capsule	Immune modulation	99.58	100.00	WP_011226326
CKHBGFBL_1	1301839	1302987	STU_RS16255	0.0	Capsule	Immune modulation	99.56	100.00	WP_041828290
CKHBGFBL_1	1297813	1298868	STU_RS16235	0.0	Capsule	Immune modulation	99.53	100.00	WP_002948022
CKHBGFBL_1	1061185	1061975	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.49	100.00	-
CKHBGFBL_1	1106609	1107478	rfbA	0.0	Capsule	Immune modulation	99.31	100.00	WP_011681286
CKHBGFBL_1	990633	991364	STR_RS05260	0.0	Capsule	Immune modulation	99.18	100.00	WP_011227248
CKHBGFBL_1	1141375	1142136	srtA	0.0	Sortase A	Exoenzyme	98.95	100.00	-
CKHBGFBL_1	984815	986230	STER_RS05250	0.0	Capsule	Immune modulation	98.52	100.00	WP_011681165
CKHBGFBL_1	990633	991364	STU_RS14645	0.0	Capsule	Immune modulation	98.50	100.00	WP_011226048
CKHBGFBL_1	989182	989922	STER_RS05305	0.0	Capsule	Immune modulation	98.11	100.00	WP_011681174
CKHBGFBL_1	989182	989922	STU_RS14635	0.0	Capsule	Immune modulation	97.98	100.00	WP_011226046
CKHBGFBL_1	990633	991364	STER_RS05315	0.0	Capsule	Immune modulation	97.95	100.00	WP_011681176
CKHBGFBL_1	991365	992825	STER_RS05320	0.0	Capsule	Immune modulation	97.74	100.00	WP_011681177
CKHBGFBL_1	1304109	1305380	STU_RS16265	0.0	Capsule	Immune modulation	97.56	99.76	WP_011226331
CKHBGFBL_1	989187	989922	STR_RS05250	0.0	Capsule	Immune modulation	97.55	98.13	WP_011227246
CKHBGFBL_1	991365	992825	STR_RS05265	0.0	Capsule	Immune modulation	97.26	100.21	WP_041827043
CKHBGFBL_1	991365	992825	STU_RS14650	0.0	Capsule	Immune modulation	97.26	100.21	WP_011226049
CKHBGFBL_1	987757	989124	STU_RS14630	0.0	Capsule	Immune modulation	94.81	100.00	WP_011226045
CKHBGFBL_1	987759	989124	STER_RS05300	0.0	Capsule	Immune modulation	94.36	99.85	WP_011681173
CKHBGFBL_1	973937	974928	STU_RS14625*	0.0	Capsule	Immune modulation	91.39	99.70	-
CKHBGFBL_1	989932	990624	STER_RS05310	0.0	Capsule	Immune modulation	89.90	100.00	WP_011681175
CKHBGFBL_1	989932	990624	STU_RS14640	0.0	Capsule	Immune modulation	88.46	100.00	WP_011226047
CKHBGFBL_1	604071	605374	eno	0.0	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
CKHBGFBL_1	989932	990624	STR_RS05255	0.0	Capsule	Immune modulation	87.88	100.00	WP_011227247
CKHBGFBL_1	1589639	1590649	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
CKHBGFBL_1	1104541	1105587	rfbB	0.0	Capsule	Immune modulation	80.52	100.00	WP_009659423
CKHBGFBL_1	145124	146407	tig/ropA	0.0	Trigger factor	Stress survival	80.42	99.77	WP_000107753
CKHBGFBL_1	973486	973928	STU_RS14620	1.09e-121	Capsule	Immune modulation	84.88	98.44	WP_011226042

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
CKHBGFBL_1	1144823	1145806	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
CKHBGFBL_1	706444	707517	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
CKHBGFBL_1	521829	522797	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
CKHBGFBL_1	1464231	1466534	pfl2(Sp_0459)	0.0	formate acetyltransferase	93.10	99.22	AAK74619
CKHBGFBL_1	726966	729212	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
CKHBGFBL_1	1209884	1211365	SPD_0310	0.0	UPF0371 protein SPD_0310	91.90	100.00	ABJ54480
CKHBGFBL_1	63237	64229	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
CKHBGFBL_1	27124	28095	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
CKHBGFBL_1	1350344	1349193	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
CKHBGFBL_1	1322332	1320344	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
CKHBGFBL_1	1128627	1126897	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.69	100.00	AAK34199
CKHBGFBL_1	1770722	1769244	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
CKHBGFBL_1	604071	605372	Eno	0.0	enolase	86.21	99.77	ABP90468
CKHBGFBL_1	1755987	1754095	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
CKHBGFBL_1	732137	730428	PGM	0.0	phosphoglucomutase	83.54	99.65	ACH87041
CKHBGFBL_1	1631340	1632350	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
CKHBGFBL_1	894433	893072	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
CKHBGFBL_1	1681660	1679768	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
CKHBGFBL_1	589641	590531	bglB	5.25e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
CKHBGFBL_1	790159	789224	SP_0676	6.97e-168	transcription facot	81.73	96.89	AAK74821
CKHBGFBL_1	729404	730261	metF	1.65e-163	methionine biosynthesis enzymes	86.01	99.31	AAK74739
CKHBGFBL_1	1029534	1028830	covR	1.03e-130	responder protein	85.11	100.00	AAK71319
CKHBGFBL_1	1190916	1190245	rr01	2.83e-125	response regulator	84.38	99.56	CAB54566
CKHBGFBL_1	1029534	1028830	walR	5.14e-121	DNA-binding response regulator	81.28	100.00	ANC99504
CKHBGFBL_1	736090	736746	CiaR	1.62e-119	two-component response regulator	86.76	97.77	AAK74935
CKHBGFBL_1	294053	294739	CovR	9.67e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
CKHBGFBL_1	736090	736746	ciaR	3.23e-117	two component system response regulator	84.93	96.90	AIK75708
CKHBGFBL_1	64526	64921	SpxA2	3.56e-74	transcriptional regulator	90.91	100.00	ERL21461
CKHBGFBL_1	146565	147065	RopE (M5005_Spy_1611)	4.73e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
CKHBGFBL_1	1464231	1466528	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	83.07	98.08	MYN69560
CKHBGFBL_1	1667202	1666183	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
CKHBGFBL_2	1	949	rep37	949 / 948	99.47	AY250830

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
CKHBGFBL_1	0.0	1.0	0.0
CKHBGFBL_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found