safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	33	29	43.93

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
ONPOEBLF_1	43663	44502	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
ONPOEBLF_1	1322334	1323185	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
ONPOEBLF_1	1133258	1134019	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
ONPOEBLF_1	974389	975312	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
ONPOEBLF_1	975272	976222	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
ONPOEBLF_1	976247	977329	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
ONPOEBLF_1	977419	978393	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
ONPOEBLF_1	978399	978878	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
ONPOEBLF_1	979071	980189	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
ONPOEBLF_1	981880	982479	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
ONPOEBLF_1	984076	984807	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
ONPOEBLF_1	1097901	1098494	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
ONPOEBLF_1	1309856	1311601	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
ONPOEBLF_1	1311603	1313303	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
ONPOEBLF_1	1314523	1315329	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
ONPOEBLF_1	1315329	1316270	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
ONPOEBLF_1	1316267	1317415	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
ONPOEBLF_1	862749	864407	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
ONPOEBLF_1	972960	974381	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
ONPOEBLF_1	984808	986262	STR_RS05265	0.0	Capsule	Immune modulation	99.93	100.00	WP_041827043
ONPOEBLF_1	1313321	1314523	STR_RS06895	0.0	Capsule	Immune modulation	99.92	100.00	WP_011227428
ONPOEBLF_1	980757	981716	STR_RS05240	0.0	Capsule	Immune modulation	99.90	100.00	WP_041827042
ONPOEBLF_1	982616	983365	STR_RS05250	0.0	Capsule	Immune modulation	99.87	100.00	WP_011227246
ONPOEBLF_1	983375	984067	STR_RS05255	0.0	Capsule	Immune modulation	99.86	100.00	WP_011227247
ONPOEBLF_1	1097901	1098494	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
ONPOEBLF_1	1097901	1098494	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
ONPOEBLF_1	1311603	1313303	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
ONPOEBLF_1	983375	984067	STU_RS14640	0.0	Capsule	Immune modulation	99.57	100.00	WP_011226047
ONPOEBLF_1	1313321	1314523	STER_RS07055	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681436
ONPOEBLF_1	1314523	1315329	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
ONPOEBLF_1	1315329	1316270	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
ONPOEBLF_1	1051845	1052635	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
ONPOEBLF_1	1316267	1317415	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
ONPOEBLF_1	1098494	1099363	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
ONPOEBLF_1	984076	984807	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
ONPOEBLF_1	982630	983365	STER_RS05305	0.0	Capsule	Immune modulation	98.37	99.33	WP_011681174
ONPOEBLF_1	984076	984807	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
ONPOEBLF_1	1309856	1311601	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
ONPOEBLF_1	984808	986262	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
ONPOEBLF_1	982630	983365	STU_RS14635	0.0	Capsule	Immune modulation	97.83	99.33	WP_011226046
ONPOEBLF_1	1320954	1322213	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
ONPOEBLF_1	984808	986262	STER_RS05320	0.0	Capsule	Immune modulation	96.99	99.79	WP_011681177
ONPOEBLF_1	983375	984067	STER_RS05310	0.0	Capsule	Immune modulation	89.75	100.00	WP_011681175
ONPOEBLF_1	605776	607079	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.54	WP_000022832
ONPOEBLF_1	1605286	1606296	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
ONPOEBLF_1	1314523	1315329	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
ONPOEBLF_1	149037	150320	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
ONPOEBLF_1	1096426	1097472	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
ONPOEBLF_1	1320259	1320957	STER_RS07095	2.95e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
ONPOEBLF_1	980465	980701	STR_RS05235*	6.65e-119	Capsule	Immune modulation	100.00	100.00	-
ONPOEBLF_1	970552	971266	waaB	0.0	Capsule	Immune modulation	100.00	67.52	WP_157822584
ONPOEBLF_1	1319439	1319905	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
ONPOEBLF_1	1313786	1314523	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
ONPOEBLF_1	1136706	1137689	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
ONPOEBLF_1	715398	716471	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
ONPOEBLF_1	523540	524508	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
ONPOEBLF_1	1484519	1486822	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
ONPOEBLF_1	1223569	1225050	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
ONPOEBLF_1	735921	738167	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
ONPOEBLF_1	63294	64286	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
ONPOEBLF_1	27181	28152	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
ONPOEBLF_1	1370688	1369537	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
ONPOEBLF_1	1336215	1334233	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
ONPOEBLF_1	1120512	1118782	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
ONPOEBLF_1	1797342	1795864	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
ONPOEBLF_1	605776	607077	Eno	0.0	enolase	86.21	99.77	ABP90468
ONPOEBLF_1	1782606	1780714	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
ONPOEBLF_1	741092	739383	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
ONPOEBLF_1	1646980	1647990	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
ONPOEBLF_1	891063	889702	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
ONPOEBLF_1	1698555	1696663	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
ONPOEBLF_1	591346	592236	bglB	2.84e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
ONPOEBLF_1	801735	800800	SP_0676	7.21e-168	transcription facot	81.73	96.89	AAK74821
ONPOEBLF_1	738359	739216	metF	8.44e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
ONPOEBLF_1	1022900	1022196	covR	3.42e-130	responder protein	84.68	100.00	AAK71319
ONPOEBLF_1	1189888	1189217	rr01	1.17e-125	response regulator	84.38	99.56	CAB54566
ONPOEBLF_1	1022900	1022196	walR	2.45e-120	DNA-binding response regulator	80.85	100.00	ANC99504
ONPOEBLF_1	309220	309906	CovR	9.82e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
ONPOEBLF_1	745045	745695	CiaR	1.35e-116	two-component response regulator	86.17	96.88	AAK74935
ONPOEBLF_1	745045	745695	ciaR	6.95e-114	two component system response regulator	83.87	96.02	AIK75708
ONPOEBLF_1	64583	64978	SpxA2	3.61e-74	transcriptional regulator	90.91	100.00	ERL21461
ONPOEBLF_1	902421	902822	SpxA1	3.67e-66	transcriptional regulator	80.60	100.00	ERL19273
ONPOEBLF_1	150478	150978	RopE (M5005_Spy_1611)	9.64e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
ONPOEBLF_1	1484519	1486816	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
ONPOEBLF_1	1684095	1683076	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
ONPOEBLF_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found