Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GLIELBNH_1 | 2736114 | 2736968 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| GLIELBNH_1 | 2716922 | 2718124 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| GLIELBNH_1 | 2487270 | 2488439 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| GLIELBNH_1 | 1348607 | 1348924 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| GLIELBNH_1 | 1348269 | 1348607 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| GLIELBNH_1 | 885013 | 887583 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| GLIELBNH_1 | 592720 | 594363 | vmlR | ARO:3004476 | vmlR | 98.90 | 100.00 | NC_000964.3 |
| GLIELBNH_1 | 337397 | 337990 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| GLIELBNH_1 | 286836 | 288269 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| GLIELBNH_1 | 274019 | 274939 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GLIELBNH_1 | 4233290 | 4234666 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.77 | 100.00 | D12567 |
| GLIELBNH_1 | 2736116 | 2736968 | aadK | Streptomycin | 98.12 | 99.77 | M26879 |
| GLIELBNH_1 | 274019 | 274939 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GLIELBNH_1 | 274019 | 274936 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| GLIELBNH_1 | 592723 | 594363 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.90 | 100.00 | WP_003234144.1 |
| GLIELBNH_1 | 4233293 | 4234666 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| GLIELBNH_1 | 2736117 | 2736968 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| GLIELBNH_1 | 4230769 | 4231287 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| GLIELBNH_1 | 2045866 | 2048463 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GLIELBNH_1 | 2288111 | 2288752 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| GLIELBNH_1 | 3751835 | 3753016 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| GLIELBNH_1 | 3750210 | 3751352 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| GLIELBNH_1 | 3337473 | 3338258 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| GLIELBNH_1 | 3751371 | 3751820 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| GLIELBNH_1 | 3336251 | 3337447 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| GLIELBNH_1 | 1995785 | 1997548 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| GLIELBNH_1 | 3334603 | 3336222 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.70 | 100.00 | NP_391078 |
| GLIELBNH_1 | 3333637 | 3334575 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| GLIELBNH_1 | 3326481 | 3333617 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391076 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GLIELBNH_1 | 132404 | 133540 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| GLIELBNH_1 | 18665 | 19534 | pdxS | 3.17e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| GLIELBNH_1 | 1658407 | 1659183 | CodY | 3.46e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GLIELBNH_1 | 3590475 | 3591044 | ClpP | 2.56e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GLIELBNH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GLIELBNH_1 | 1290970 | 1318785 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| GLIELBNH_1 | 2170488 | 2181410 | False | Siphoviridae | VOG0918, VOG4551, VOG0025, VOG7476, VOG0322, VOG6211, VOG0608, VOG4908 |
| GLIELBNH_1 | 2235621 | 2259816 | False | Siphoviridae | VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG4705, VOG8610, VOG7930, VOG0275, VOG9667, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG7900, VOG4574, VOG8537 |
| GLIELBNH_1 | 2665450 | 2683762 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749 |
| GLIELBNH_1 | 3049906 | 3076637 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841, VOG6422, VOG0198, VOG1884, VOG0025, VOG9600 |
Phigaro v2.4.0 (Database: Jan. 2024 release)