Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ELLDBLJA_1 | 1653507 | 1653845 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| ELLDBLJA_1 | 1653190 | 1653507 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| ELLDBLJA_1 | 668927 | 670096 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| ELLDBLJA_1 | 494621 | 495823 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| ELLDBLJA_1 | 476226 | 477080 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| ELLDBLJA_3 | 360039 | 360959 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| ELLDBLJA_3 | 346706 | 348145 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| ELLDBLJA_3 | 297051 | 297644 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| ELLDBLJA_3 | 29189 | 30832 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| ELLDBLJA_5 | 27478 | 30048 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ELLDBLJA_3 | 360039 | 360959 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| ELLDBLJA_2 | 627985 | 629361 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
| ELLDBLJA_1 | 476226 | 477080 | aadK | Streptomycin | 98.48 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ELLDBLJA_3 | 360042 | 360959 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| ELLDBLJA_2 | 625465 | 625983 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| ELLDBLJA_2 | 627988 | 629361 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| ELLDBLJA_3 | 29189 | 30829 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| ELLDBLJA_1 | 476226 | 477077 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| ELLDBLJA_1 | 976411 | 979005 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ELLDBLJA_2 | 142949 | 144091 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| ELLDBLJA_2 | 144574 | 145755 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| ELLDBLJA_2 | 144110 | 144559 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| ELLDBLJA_1 | 1023905 | 1025668 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| ELLDBLJA_4 | 266299 | 267237 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| ELLDBLJA_4 | 262616 | 263401 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| ELLDBLJA_1 | 868810 | 869451 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| ELLDBLJA_4 | 267257 | 274393 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.14 | 100.00 | NP_391076 |
| ELLDBLJA_4 | 263427 | 264623 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| ELLDBLJA_4 | 264652 | 266271 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.95 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ELLDBLJA_7 | 31783 | 32919 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ELLDBLJA_11 | 4376 | 5245 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| ELLDBLJA_1 | 1340243 | 1339467 | CodY | 1.69e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ELLDBLJA_4 | 8672 | 8103 | ClpP | 2.26e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| ELLDBLJA_1 | 1802671 | 1802279 | spxA1 | 1.33e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| ELLDBLJA_1 | 1802671 | 1802279 | spx | 7.91e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| ELLDBLJA_8 | 20900 | 21142 | SpoVG | 7.56e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| ELLDBLJA_3 | 77542 | 77345 | CspA | 1.99e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ELLDBLJA_1 | 0.0 | 1.0 | 0.0 |
| ELLDBLJA_2 | 0.0 | 1.0 | 0.0 |
| ELLDBLJA_3 | 0.0 | 1.0 | 0.0 |
| ELLDBLJA_4 | 0.0 | 1.0 | 0.0 |
| ELLDBLJA_5 | 0.0 | 1.0 | 0.0 |
| ELLDBLJA_6 | 0.0 | 1.0 | 0.0 |
| ELLDBLJA_7 | 0.0 | 1.0 | 0.0 |
| ELLDBLJA_8 | 0.0 | 1.0 | 0.0 |
| ELLDBLJA_9 | 0.0 | 1.0 | 0.0 |
| ELLDBLJA_10 | 1.0 | 0.0 | 1.0 |
| ELLDBLJA_11 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ELLDBLJA_1 | 315539 | 349789 | False | Siphoviridae | VOG7438, VOG1047, VOG0021, VOG1565, VOG0026, VOG4609, VOG0327, VOG4548, VOG0198, VOG4841, VOG4581, VOG1886, VOG3644, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG4545, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG5008, VOG0641, VOG4574 |
| ELLDBLJA_1 | 1683684 | 1709795 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| ELLDBLJA_9 | 13718 | 43662 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG6005, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309 |
| ELLDBLJA_10 | 228 | 16067 | False | Siphoviridae | VOG4605, VOG4545, VOG0701, VOG10044, VOG6548, VOG0697, VOG0696, VOG0695, VOG5007, VOG2621, VOG4572, VOG9317, VOG5615, VOG0692, VOG0691, VOG0612 |
| ELLDBLJA_10 | 20216 | 25899 | False | Unknown | VOG5090, VOG6587, VOG0102, VOG0189, VOG11060, VOG0283, VOG0535, VOG1879, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)