Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IFKKFKGI_3 | 634353 | 635522 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| IFKKFKGI_3 | 464224 | 465426 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| IFKKFKGI_3 | 445835 | 446689 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| IFKKFKGI_2 | 427250 | 427567 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IFKKFKGI_2 | 426912 | 427250 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IFKKFKGI_4 | 335235 | 336155 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| IFKKFKGI_4 | 321908 | 323341 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| IFKKFKGI_4 | 272264 | 272857 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| IFKKFKGI_4 | 29203 | 30846 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| IFKKFKGI_5 | 27451 | 30021 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IFKKFKGI_1 | 1007616 | 1008992 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | D12567 |
| IFKKFKGI_3 | 445835 | 446687 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| IFKKFKGI_4 | 335235 | 336155 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IFKKFKGI_1 | 1007619 | 1008992 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_063856081.1 |
| IFKKFKGI_4 | 29203 | 30843 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| IFKKFKGI_3 | 445835 | 446686 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| IFKKFKGI_4 | 335238 | 336155 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| IFKKFKGI_1 | 1005118 | 1005636 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| IFKKFKGI_3 | 930509 | 933106 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IFKKFKGI_1 | 109224 | 110162 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| IFKKFKGI_1 | 521503 | 522684 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IFKKFKGI_1 | 110190 | 111809 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| IFKKFKGI_1 | 113060 | 113845 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| IFKKFKGI_3 | 836319 | 836960 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| IFKKFKGI_1 | 111838 | 113034 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| IFKKFKGI_1 | 102068 | 109204 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.99 | 100.00 | NP_391076 |
| IFKKFKGI_1 | 519878 | 521020 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| IFKKFKGI_3 | 978284 | 980047 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| IFKKFKGI_1 | 521039 | 521488 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IFKKFKGI_8 | 28138 | 27002 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IFKKFKGI_11 | 11333 | 10464 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IFKKFKGI_2 | 737268 | 738044 | CodY | 8.39e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IFKKFKGI_1 | 380097 | 380666 | ClpP | 6.28e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IFKKFKGI_2 | 276450 | 276842 | spxA1 | 6.59e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| IFKKFKGI_2 | 276450 | 276842 | spx | 3.93e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| IFKKFKGI_10 | 34796 | 34554 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| IFKKFKGI_4 | 77595 | 77398 | CspA | 1.86e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IFKKFKGI_1 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_2 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_3 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_4 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_5 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_6 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_7 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_8 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_9 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_10 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_11 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_12 | 0.0 | 1.0 | 0.0 |
| IFKKFKGI_16 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IFKKFKGI_2 | 370949 | 397432 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| IFKKFKGI_6 | 30118 | 35280 | False | Unknown | VOG4551, VOG0025, VOG7476, VOG0322 |
| IFKKFKGI_9 | 21024 | 46999 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG10989, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4673 |
Phigaro v2.4.0 (Database: Jan. 2024 release)