Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PFBLLPIH_1 | 2587517 | 2588371 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| PFBLLPIH_1 | 2568773 | 2569975 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| PFBLLPIH_1 | 2341716 | 2342885 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| PFBLLPIH_1 | 1353158 | 1353475 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| PFBLLPIH_1 | 1352820 | 1353158 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| PFBLLPIH_1 | 893168 | 895738 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| PFBLLPIH_1 | 581240 | 582883 | vmlR | ARO:3004476 | vmlR | 98.17 | 100.00 | NC_000964.3 |
| PFBLLPIH_1 | 337768 | 338361 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| PFBLLPIH_1 | 287209 | 288642 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.00 | JYFL01000006.1 |
| PFBLLPIH_1 | 274396 | 275316 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| PFBLLPIH_1 | 274396 | 275316 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| PFBLLPIH_1 | 4060614 | 4061990 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.42 | 100.00 | X08034 |
| PFBLLPIH_1 | 2587519 | 2588371 | aadK | Streptomycin | 98.94 | 99.77 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PFBLLPIH_1 | 274396 | 275313 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| PFBLLPIH_1 | 4060617 | 4061990 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_003242953.1 |
| PFBLLPIH_1 | 2587520 | 2588371 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| PFBLLPIH_1 | 581243 | 582883 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.17 | 100.00 | WP_003234144.1 |
| PFBLLPIH_1 | 4058115 | 4058633 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 89.60 | 100.00 | WP_003242546.1 |
| PFBLLPIH_1 | 2032796 | 2035390 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.03 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PFBLLPIH_1 | 2142349 | 2142990 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| PFBLLPIH_1 | 3132647 | 3139783 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.96 | 100.00 | NP_391076 |
| PFBLLPIH_1 | 3139803 | 3140741 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| PFBLLPIH_1 | 3140769 | 3142388 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.81 | 100.00 | NP_391078 |
| PFBLLPIH_1 | 3551784 | 3552965 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| PFBLLPIH_1 | 1982409 | 1984172 | dep/capD | 0.0 | Capsule | Immune modulation | 99.60 | 100.00 | NP_389723 |
| PFBLLPIH_1 | 3551320 | 3551769 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| PFBLLPIH_1 | 3143639 | 3144424 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| PFBLLPIH_1 | 3550159 | 3551301 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| PFBLLPIH_1 | 3142417 | 3143613 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PFBLLPIH_1 | 132397 | 133533 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| PFBLLPIH_1 | 18665 | 19534 | pdxS | 3.04e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| PFBLLPIH_1 | 1666513 | 1667289 | CodY | 3.32e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| PFBLLPIH_1 | 3398572 | 3399141 | ClpP | 2.46e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PFBLLPIH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| PFBLLPIH_1 | 1296908 | 1323340 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| PFBLLPIH_1 | 2518787 | 2538014 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG10095, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685 |
Phigaro v2.4.0 (Database: Jan. 2024 release)