Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 8 | 10 | 4 | 13.42 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HBHHAHAJ_1 | 2388376 | 2389545 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| HBHHAHAJ_1 | 1371279 | 1371596 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| HBHHAHAJ_1 | 1370941 | 1371279 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| HBHHAHAJ_1 | 908456 | 911026 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| HBHHAHAJ_1 | 348491 | 349084 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| HBHHAHAJ_1 | 296717 | 298150 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HBHHAHAJ_1 | 286717 | 287638 | mph(K) | Spiramycin, Telithromycin | 97.18 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HBHHAHAJ_1 | 4124887 | 4125405 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| HBHHAHAJ_1 | 286717 | 287340 | mphK | macrolide 2'-phosphotransferase MphK | PARTIALX | 98.08 | 67.97 | WP_003246254.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HBHHAHAJ_1 | 3631652 | 3632833 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| HBHHAHAJ_1 | 3207824 | 3208609 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| HBHHAHAJ_1 | 2032971 | 2034734 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| HBHHAHAJ_1 | 3630027 | 3631169 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
| HBHHAHAJ_1 | 3203999 | 3204937 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| HBHHAHAJ_1 | 3631188 | 3631637 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| HBHHAHAJ_1 | 3206602 | 3207798 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.41 | 100.00 | NP_391079 |
| HBHHAHAJ_1 | 3196843 | 3203979 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.98 | 100.00 | NP_391076 |
| HBHHAHAJ_1 | 2181716 | 2182357 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.97 | 100.00 | NP_390062 |
| HBHHAHAJ_1 | 3204965 | 3206584 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HBHHAHAJ_1 | 134118 | 135260 | tufA | 0.0 | elongation factor Tu | 85.56 | 96.19 | AHM69299 |
| HBHHAHAJ_1 | 18663 | 19532 | pdxS | 3.08e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| HBHHAHAJ_1 | 1700257 | 1701033 | CodY | 3.37e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HBHHAHAJ_1 | 3473380 | 3473949 | ClpP | 2.50e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HBHHAHAJ_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HBHHAHAJ_1 | 1330607 | 1340216 | False | Myoviridae / Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG4691, VOG4862, VOG7718, VOG7718, VOG3390 |
| HBHHAHAJ_1 | 1824886 | 1844054 | False | Unknown | VOG10230, VOG7900, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG8610, VOG4705 |
| HBHHAHAJ_1 | 2573910 | 2597618 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796 |
| HBHHAHAJ_1 | 2914938 | 2940708 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841, VOG0198, VOG1884, VOG0025, VOG9600 |
Phigaro v2.4.0 (Database: Jan. 2024 release)