Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BAKBDIAH_1 | 2706377 | 2707231 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| BAKBDIAH_1 | 2689173 | 2690375 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| BAKBDIAH_1 | 2498359 | 2499528 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BAKBDIAH_1 | 1431646 | 1431963 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BAKBDIAH_1 | 1431308 | 1431646 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BAKBDIAH_1 | 970633 | 973203 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| BAKBDIAH_1 | 633734 | 635380 | vmlR | ARO:3004476 | vmlR | 98.91 | 100.18 | NC_000964.3 |
| BAKBDIAH_1 | 383463 | 384056 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| BAKBDIAH_1 | 332967 | 334400 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| BAKBDIAH_1 | 320158 | 321078 | mphK | ARO:3004541 | mphK | 96.41 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BAKBDIAH_1 | 2706379 | 2707231 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| BAKBDIAH_1 | 320158 | 321078 | mph(K) | Spiramycin, Telithromycin | 96.75 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BAKBDIAH_1 | 633737 | 635380 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.91 | 100.00 | WP_003234144.1 |
| BAKBDIAH_1 | 2706380 | 2707231 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| BAKBDIAH_1 | 320158 | 321075 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.41 | 100.00 | WP_003246254.1 |
| BAKBDIAH_1 | 4220708 | 4221226 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| BAKBDIAH_1 | 2162427 | 2165024 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.71 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BAKBDIAH_1 | 2301027 | 2301668 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| BAKBDIAH_1 | 3717625 | 3718806 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| BAKBDIAH_1 | 3294639 | 3295424 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| BAKBDIAH_1 | 3717161 | 3717610 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| BAKBDIAH_1 | 3293417 | 3294613 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| BAKBDIAH_1 | 3716000 | 3717142 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| BAKBDIAH_1 | 2116748 | 2118511 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| BAKBDIAH_1 | 3283647 | 3290783 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.56 | 100.00 | NP_391076 |
| BAKBDIAH_1 | 3290803 | 3291741 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
| BAKBDIAH_1 | 3291769 | 3293388 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BAKBDIAH_1 | 132625 | 133761 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BAKBDIAH_1 | 18681 | 19550 | pdxS | 3.16e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BAKBDIAH_1 | 1744440 | 1745216 | CodY | 3.45e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BAKBDIAH_1 | 3557972 | 3558541 | ClpP | 2.56e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BAKBDIAH_2 | 67 | 253 | Col(SD853) | 187 / 194 | 88.24 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BAKBDIAH_1 | 0.0 | 1.0 | 0.0 |
| BAKBDIAH_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BAKBDIAH_1 | 169108 | 177225 | False | Siphoviridae | VOG8241, VOG3549, VOG1878, VOG0083, VOG1309, VOG1292, VOG11060, VOG3709, VOG0025, VOG10041 |
| BAKBDIAH_1 | 187334 | 211553 | False | Myoviridae | VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862, VOG4612, VOG3390, VOG4705 |
| BAKBDIAH_1 | 1376738 | 1402034 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG7895, VOG0753 |
| BAKBDIAH_1 | 3158155 | 3180771 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG3480, VOG1329, VOG4555, VOG4564, VOG0720, VOG0796 |
| BAKBDIAH_1 | 3190297 | 3193990 | False | Unknown | VOG4600, VOG4552, VOG8241 |
Phigaro v2.4.0 (Database: Jan. 2024 release)