safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
DJOGKOEH_1	2623162	2624016	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
DJOGKOEH_1	2604457	2605659	blt	ARO:3003006	blt	100.00	100.00	L32599.1
DJOGKOEH_1	2382138	2383307	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
DJOGKOEH_1	1358019	1358336	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
DJOGKOEH_1	1357681	1358019	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
DJOGKOEH_1	897952	900522	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
DJOGKOEH_1	583822	585465	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
DJOGKOEH_1	338755	339348	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
DJOGKOEH_1	288252	289685	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
DJOGKOEH_1	275437	276357	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
DJOGKOEH_1	2623162	2624016	aadK	Streptomycin	100.00	100.00	M26879
DJOGKOEH_1	275437	276357	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
DJOGKOEH_1	4075186	4076562	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
DJOGKOEH_1	2623165	2624016	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
DJOGKOEH_1	275437	276354	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
DJOGKOEH_1	4072668	4073186	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
DJOGKOEH_1	4075189	4076562	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
DJOGKOEH_1	583825	585465	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
DJOGKOEH_1	2073226	2075820	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
DJOGKOEH_1	3585327	3586469	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
DJOGKOEH_1	3586952	3588133	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
DJOGKOEH_1	3586488	3586937	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
DJOGKOEH_1	2026568	2028331	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
DJOGKOEH_1	3178992	3179777	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
DJOGKOEH_1	2182792	2183433	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062
DJOGKOEH_1	3168000	3175136	dhbF	Bacillibactin	Nutritional/Metabolic factor	99.99	100.00	NP_391076
DJOGKOEH_1	3175156	3176094	dhbB	Bacillibactin	Nutritional/Metabolic factor	99.89	100.00	NP_391077
DJOGKOEH_1	3177770	3178966	dhbC	Bacillibactin	Nutritional/Metabolic factor	99.67	100.00	NP_391079
DJOGKOEH_1	3176122	3177741	dhbE	Bacillibactin	Nutritional/Metabolic factor	99.57	100.00	NP_391078

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
DJOGKOEH_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
DJOGKOEH_1	18665	19534	pdxS	3.06e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
DJOGKOEH_1	1671010	1671786	CodY	3.35e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
DJOGKOEH_1	3433715	3434284	ClpP	2.48e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
DJOGKOEH_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
DJOGKOEH_1	1301734	1327842	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
DJOGKOEH_1	1861958	1897532	False	Siphoviridae	VOG4602, VOG9667, VOG5353, VOG0181, VOG4552, VOG4566, VOG9600, VOG0025, VOG1884, VOG0198, VOG6093, VOG7462, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641, VOG4918
DJOGKOEH_1	2554528	2584772	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)