Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DILPPBME_1 | 2678600 | 2679454 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| DILPPBME_1 | 2659895 | 2661097 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| DILPPBME_1 | 2437576 | 2438745 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DILPPBME_1 | 1413209 | 1413526 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| DILPPBME_1 | 1412871 | 1413209 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DILPPBME_1 | 953130 | 955700 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| DILPPBME_1 | 639000 | 640643 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| DILPPBME_1 | 393933 | 394526 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| DILPPBME_1 | 343430 | 344863 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| DILPPBME_1 | 330615 | 331535 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DILPPBME_1 | 2678600 | 2679454 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| DILPPBME_1 | 330615 | 331535 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| DILPPBME_1 | 4130628 | 4132004 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DILPPBME_1 | 2678603 | 2679454 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| DILPPBME_1 | 330615 | 331532 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| DILPPBME_1 | 4128110 | 4128628 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| DILPPBME_1 | 4130631 | 4132004 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| DILPPBME_1 | 639003 | 640643 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| DILPPBME_1 | 2128663 | 2131257 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DILPPBME_1 | 3640769 | 3641911 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| DILPPBME_1 | 3642394 | 3643575 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| DILPPBME_1 | 3641930 | 3642379 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| DILPPBME_1 | 2082005 | 2083768 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| DILPPBME_1 | 3234434 | 3235219 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| DILPPBME_1 | 2238230 | 2238871 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| DILPPBME_1 | 3223442 | 3230578 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.99 | 100.00 | NP_391076 |
| DILPPBME_1 | 3230598 | 3231536 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| DILPPBME_1 | 3233212 | 3234408 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.67 | 100.00 | NP_391079 |
| DILPPBME_1 | 3231564 | 3233183 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DILPPBME_1 | 132473 | 133609 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DILPPBME_1 | 18665 | 19534 | pdxS | 3.09e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DILPPBME_1 | 1726493 | 1727269 | CodY | 3.37e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DILPPBME_1 | 3489157 | 3489726 | ClpP | 2.50e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| DILPPBME_3 | 4643 | 4829 | Col(SD853) | 187 / 194 | 88.24 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DILPPBME_1 | 0.0 | 1.0 | 0.0 |
| DILPPBME_2 | 1.0 | 0.0 | 1.0 |
| DILPPBME_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DILPPBME_1 | 168741 | 175820 | False | Siphoviridae | VOG4603, VOG8241, VOG3549, VOG1878, VOG0083, VOG1309, VOG1292, VOG11060 |
| DILPPBME_1 | 187168 | 213061 | False | Myoviridae | VOG1328, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862, VOG0906, VOG7718, VOG3390, VOG7895 |
| DILPPBME_1 | 1356924 | 1383032 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DILPPBME_1 | 1917441 | 1952973 | False | Siphoviridae | VOG4602, VOG9667, VOG5353, VOG0181, VOG4552, VOG4566, VOG9600, VOG0025, VOG1884, VOG0198, VOG6093, VOG7462, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641, VOG4918 |
| DILPPBME_1 | 2609966 | 2640210 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
| DILPPBME_2 | 34745 | 52200 | False | Siphoviridae | VOG2352, VOG0397, VOG1198, VOG4561, VOG4714, VOG2352, VOG3851, VOG2725, VOG4549, VOG5292, VOG1574, VOG0918, VOG4551 |
| DILPPBME_2 | 69326 | 82645 | False | Siphoviridae | VOG4724, VOG0018, VOG9096, VOG0221, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539 |
Phigaro v2.4.0 (Database: Jan. 2024 release)