Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JGLMLABP_1 | 2738812 | 2739666 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| JGLMLABP_1 | 2720075 | 2721277 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| JGLMLABP_1 | 2494304 | 2495473 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| JGLMLABP_1 | 1386727 | 1387044 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| JGLMLABP_1 | 1386389 | 1386727 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| JGLMLABP_1 | 925049 | 927619 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| JGLMLABP_1 | 628513 | 630156 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| JGLMLABP_1 | 386501 | 387094 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| JGLMLABP_1 | 336017 | 337450 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| JGLMLABP_1 | 323203 | 324123 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| JGLMLABP_1 | 4198022 | 4199398 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | D12567 |
| JGLMLABP_1 | 2738814 | 2739666 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| JGLMLABP_1 | 323203 | 324123 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JGLMLABP_1 | 4198025 | 4199398 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_063856081.1 |
| JGLMLABP_1 | 628516 | 630156 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| JGLMLABP_1 | 2738815 | 2739666 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| JGLMLABP_1 | 323203 | 324120 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| JGLMLABP_1 | 4195524 | 4196042 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| JGLMLABP_1 | 2066620 | 2069217 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JGLMLABP_1 | 3299629 | 3300567 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| JGLMLABP_1 | 3711909 | 3713090 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| JGLMLABP_1 | 3300595 | 3302214 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| JGLMLABP_1 | 3303465 | 3304250 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| JGLMLABP_1 | 2292866 | 2293507 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| JGLMLABP_1 | 3302243 | 3303439 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| JGLMLABP_1 | 3292473 | 3299609 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391076 |
| JGLMLABP_1 | 3710284 | 3711426 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| JGLMLABP_1 | 2019679 | 2021442 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| JGLMLABP_1 | 3711445 | 3711894 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JGLMLABP_1 | 132477 | 133613 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| JGLMLABP_1 | 18665 | 19534 | pdxS | 3.14e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| JGLMLABP_1 | 1696745 | 1697521 | CodY | 3.43e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| JGLMLABP_1 | 3570503 | 3571072 | ClpP | 2.54e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JGLMLABP_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JGLMLABP_1 | 169130 | 174720 | False | Unknown | VOG8241, VOG3549, VOG1878, VOG0083, VOG1309 |
| JGLMLABP_1 | 184674 | 214260 | False | Myoviridae | VOG4567, VOG1328, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG10120, VOG4691, VOG4862, VOG0906, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136 |
| JGLMLABP_1 | 1330425 | 1356908 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| JGLMLABP_1 | 2185479 | 2188492 | False | Unknown | VOG0025, VOG7476 |
| JGLMLABP_1 | 2241912 | 2264240 | False | Siphoviridae | VOG7718, VOG3390, VOG4705, VOG5008, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG4660 |
| JGLMLABP_1 | 2672846 | 2703231 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG10989, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG4673, VOG4693, VOG0189, VOG11060, VOG1309 |
Phigaro v2.4.0 (Database: Jan. 2024 release)