Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FNNKMDAK_1 | 751508 | 752677 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FNNKMDAK_1 | 525755 | 526957 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| FNNKMDAK_1 | 507415 | 508269 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| FNNKMDAK_2 | 427150 | 427467 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| FNNKMDAK_2 | 426812 | 427150 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| FNNKMDAK_4 | 356974 | 358599 | vmlR | ARO:3004476 | vmlR | 98.52 | 98.90 | NC_000964.3 |
| FNNKMDAK_4 | 98549 | 99142 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| FNNKMDAK_4 | 48014 | 49453 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| FNNKMDAK_4 | 35202 | 36122 | mphK | ARO:3004541 | mphK | 97.06 | 100.00 | CP010314.1 |
| FNNKMDAK_5 | 27473 | 30043 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FNNKMDAK_7 | 57929 | 59305 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.42 | 100.00 | D12567 |
| FNNKMDAK_1 | 507415 | 508269 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| FNNKMDAK_4 | 35202 | 36122 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FNNKMDAK_7 | 57929 | 59302 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| FNNKMDAK_1 | 507415 | 508266 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| FNNKMDAK_4 | 356977 | 358599 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.52 | 98.90 | WP_003234144.1 |
| FNNKMDAK_4 | 35202 | 36119 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.06 | 100.00 | WP_003246254.1 |
| FNNKMDAK_7 | 61317 | 61835 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| FNNKMDAK_8 | 56261 | 58858 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.71 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FNNKMDAK_3 | 821683 | 823302 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.81 | 100.00 | NP_391078 |
| FNNKMDAK_3 | 823330 | 824268 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.79 | 100.00 | NP_391077 |
| FNNKMDAK_3 | 412681 | 413862 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| FNNKMDAK_8 | 9565 | 11328 | dep/capD | 0.0 | Capsule | Immune modulation | 99.60 | 100.00 | NP_389723 |
| FNNKMDAK_3 | 819647 | 820432 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| FNNKMDAK_3 | 414345 | 415487 | capA | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | NP_391469 |
| FNNKMDAK_1 | 955068 | 955709 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| FNNKMDAK_3 | 413877 | 414326 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| FNNKMDAK_3 | 824288 | 831424 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391076 |
| FNNKMDAK_3 | 820458 | 821654 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FNNKMDAK_10 | 31972 | 33108 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FNNKMDAK_12 | 11334 | 10465 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FNNKMDAK_2 | 737774 | 738550 | CodY | 7.98e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FNNKMDAK_3 | 565264 | 564695 | ClpP | 5.61e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| FNNKMDAK_2 | 276446 | 276838 | spxA1 | 2.37e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| FNNKMDAK_11 | 20814 | 21056 | SpoVG | 7.55e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| FNNKMDAK_4 | 298323 | 298520 | CspA | 1.67e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| FNNKMDAK_1 | 943107 | 942913 | CspD | 6.43e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FNNKMDAK_1 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_2 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_3 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_4 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_5 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_6 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_7 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_8 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_9 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_10 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_11 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_12 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_13 | 0.0 | 1.0 | 0.0 |
| FNNKMDAK_15 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FNNKMDAK_1 | 277315 | 294283 | False | Siphoviridae | VOG0152, VOG6168, VOG0189, VOG4907, VOG0943, VOG0025, VOG4551, VOG2215, VOG1563, VOG4134, VOG6546, VOG5753, VOG10937, VOG5385, VOG6497, VOG0026, VOG5543, VOG1093 |
| FNNKMDAK_1 | 314223 | 341934 | False | Siphoviridae | VOG6430, VOG0531, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG0753, VOG4574, VOG0602, VOG8440, VOG6116 |
| FNNKMDAK_1 | 558611 | 577322 | False | Myoviridae | VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG10989, VOG4691, VOG4862 |
| FNNKMDAK_2 | 370997 | 397318 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| FNNKMDAK_2 | 936554 | 953331 | False | Unknown | VOG4358, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG8610 |
| FNNKMDAK_6 | 24547 | 38664 | False | Siphoviridae | VOG0048, VOG0048, VOG4908, VOG6211, VOG0322, VOG7476, VOG0025, VOG4551, VOG0918 |
Phigaro v2.4.0 (Database: Jan. 2024 release)