safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	8	18.25

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BOGNBLAN_2	524193	524531	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
BOGNBLAN_2	523876	524193	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
BOGNBLAN_3	465719	466573	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
BOGNBLAN_3	447010	448212	blt	ARO:3003006	blt	100.00	100.00	L32599.1
BOGNBLAN_3	224691	225860	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
BOGNBLAN_4	162961	163554	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
BOGNBLAN_4	112458	113897	lmrB	ARO:3002813	lmrB	99.79	100.42	JYFL01000006.1
BOGNBLAN_4	99643	100563	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
BOGNBLAN_8	47462	49105	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
BOGNBLAN_5	27368	29938	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BOGNBLAN_3	465719	466573	aadK	Streptomycin	100.00	100.00	M26879
BOGNBLAN_4	99643	100563	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
BOGNBLAN_1	36389	37765	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BOGNBLAN_3	465722	466573	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
BOGNBLAN_4	99643	100560	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
BOGNBLAN_1	39765	40283	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
BOGNBLAN_1	36389	37762	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
BOGNBLAN_8	47465	49105	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
BOGNBLAN_6	168821	171415	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BOGNBLAN_1	526112	527254	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
BOGNBLAN_1	524448	525629	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
BOGNBLAN_1	525644	526093	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
BOGNBLAN_6	216310	218073	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
BOGNBLAN_1	932805	933590	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
BOGNBLAN_1	936488	937426	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
BOGNBLAN_1	933616	934812	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
BOGNBLAN_1	934841	936460	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
BOGNBLAN_1	937446	944582	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
BOGNBLAN_3	25345	25986	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BOGNBLAN_10	31775	32911	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
BOGNBLAN_14	11301	10432	pdxS	1.12e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
BOGNBLAN_2	210929	210153	CodY	7.70e-125	nutrient-responsive regulator	81.08	100.00	ACJ80679
BOGNBLAN_1	678866	678297	ClpP	6.29e-99	part of proteolytic complex	81.05	97.44	BAB94595
BOGNBLAN_2	673354	672962	spxA1	2.28e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
BOGNBLAN_11	35060	34818	SpoVG	7.56e-39	RNA-binding protein	81.48	79.41	AAT02994
BOGNBLAN_8	1990	2187	CspA	3.38e-32	cold shock protein	90.91	100.00	CAA62903
BOGNBLAN_3	37948	38142	CspD	5.56e-28	cold shock protein	81.54	98.48	CAC99957

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BOGNBLAN_1	0.0	1.0	0.0
BOGNBLAN_2	0.0	1.0	0.0
BOGNBLAN_3	0.0	1.0	0.0
BOGNBLAN_4	0.0	1.0	0.0
BOGNBLAN_5	0.0	1.0	0.0
BOGNBLAN_6	0.0	1.0	0.0
BOGNBLAN_7	1.0	0.0	1.0
BOGNBLAN_8	0.0	1.0	0.0
BOGNBLAN_9	0.0	1.0	0.0
BOGNBLAN_10	0.0	1.0	0.0
BOGNBLAN_11	0.0	1.0	0.0
BOGNBLAN_12	0.0	1.0	0.0
BOGNBLAN_13	0.0	1.0	0.0
BOGNBLAN_14	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BOGNBLAN_2	554370	580478	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667
BOGNBLAN_3	397081	427325	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600
BOGNBLAN_7	29885	42983	False	Unknown	VOG4549, VOG2725, VOG3851, VOG2352, VOG4714, VOG4561, VOG1198, VOG0397, VOG2352, VOG11080
BOGNBLAN_7	62397	82991	False	Siphoviridae	VOG3616, VOG1024, VOG10944, VOG9244, VOG4599, VOG4605, VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556

Phigaro v2.4.0 (Database: Jan. 2024 release)