GCA_003475545.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaGPGAIPCF_2 (original name was: gnl|Prokka|GPGAIPCF_2)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	NRPS,cyclic-lactone-autoinducer	24,119	70,691	HTTPCA/prepiscibactin/piscibactin	NRP+Polyketide	4%

gnlProkkaGPGAIPCF_45 (original name was: gnl|Prokka|GPGAIPCF_45)

Region	Type	From	To	Most similar known cluster		Similarity
Region 38.1	lanthipeptide-class-ii	64	23,030	xantholipin	Polyketide	6%

gnlProkkaGPGAIPCF_75 (original name was: gnl|Prokka|GPGAIPCF_75)

Region	Type	From	To	Most similar known cluster		Similarity
Region 68.1	ranthipeptide	14,685	26,245

gnlProkkaGPGAIPCF_122 (original name was: gnl|Prokka|GPGAIPCF_122)

Region	Type	From	To	Most similar known cluster		Similarity
Region 115.1	RRE-containing	1	10,463

gnlProkkaGPGAIPCF_126 (original name was: gnl|Prokka|GPGAIPCF_126)

Region	Type	From	To	Most similar known cluster		Similarity
Region 119.1	cyclic-lactone-autoinducer	1	8,836

Compact view

gnlProkkaGPGAIPCF_2 - Region 1 - NRPS,cyclic-lactone-autoinducer

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Location: 24,119 - 70,691 nt. (total: 46,573 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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core biosynthetic genes

additional biosynthetic genes

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 24118 - 70691:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg2_53

Search NORINE for peptide: strict or relaxed

AMP-binding (553..948): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSMIWK
Nearest Stachelhaus code(s):: Cys DLYNMSMIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg2_54

Search NORINE for peptide: strict or relaxed

AMP-binding (25..431): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVLSK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVLCK (79% 8Å match); Sal PLPAQGVLNK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

gnlProkkaGPGAIPCF_45 - Region 1 - lanthipeptide-class-ii

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Location: 64 - 23,030 nt. (total: 22,967 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

Lanthipeptide predictions

Lanthipeptide(s) for ctg38_13

ctg38_10

Leader:

MKTCSERNEQRINSLQLSESLEDEIKELDGKNVEAGDVIFT

Core:

TDTSSFLTIVCC

Core with dehydrations:

DhbDDhbDhaDhaFLDhbIVCysCys

antiSMASH-DB 4.0 matches for ctg38_10

100.0% NZ_CP102267.1and 2 others:

NZ_JADMOE010000006.1 Lanthipeptides: I2G55_RS05425_lanthipeptideNZ_LT635469.1 Lanthipeptides: BQ7352_RS14540_lanthipeptide

Lanthipeptides: NQ550_RS10225_lanthipeptide

1

TDTSSFLTIVCC

12

TDTSSFLTIVCC

1

TDTSSFLTIVCC

12

50.0% NZ_CP113781.1: Lanthipeptides: GGADHKLB_RS14125_lanthipeptide

2

DTSSFLTIVCC

12

+ + FLTI+CC

2

NCTHFLTILCC

12

40.0% NZ_AP025566.1: Lanthipeptides: MWL92_RS00840_lanthipeptide

4

SSFLTIVCC

12

+SF TI+CC

7

TSFCTIICC

15

with 10 more sequence matches

33.3% NZ_CP036524.1: Lanthipeptides: LAJLEIBI_RS18025_lanthipeptide

4

SSFLTIVCC

12

++F TI+CC

7

TTFFTIICC

15

31.6% NZ_CP036170.1: Lanthipeptides: HDCHBGLK_RS18795_lanthipeptide

4

SSFLTIVCC

12

+FLTI CC

11

GTFLTIYCC

19

29.2% NZ_CP048626.1: Lanthipeptides: GXM18_RS04600_lanthipeptide

2

DTSSFLTIVCC

12

D +SF TI CC

14

DNASFFTIFCC

24

26.9% NZ_CP070036.1and 3 others:

NZ_JADMPO010000043.1 Lanthipeptides: I2I27_RS16805_lanthipeptideNZ_JADNGF010000061.1 Lanthipeptides: I2E06_RS15650_lanthipeptideNZ_JAQMLK010000037.1 Lanthipeptides: PNW18_RS16660_lanthipeptide

Lanthipeptides: I6K72_RS03970_lanthipeptide

4

SSFLTIVCC

12

S+FLTI CC

18

SAFLTIYCC

26

20.6% NZ_JADPCB010000035.1: Lanthipeptides: I4W51_RS14990_lanthipeptide

3

TSSFLTIVCC

12

T +FLTI+CC

25

TGAFLTILCC

34

17.9% NZ_CP094681.1: Lanthipeptides: K5I26_RS05295_lanthipeptide

1

TDTSSFLTIVCC

12

+D + FLTI CC

28

SDCALFLTIFCC

39

17.9% NZ_JAOQJV010000004.1: Lanthipeptides: OCV65_RS05205_lanthipeptide

4

SSFLTIVCC

12

S FLTI+CC

31

SGFLTIICC

39

17.6% NZ_MCGI01000004.1and 1 other:

NZ_MPDJ01000004.1 Lanthipeptides: BLA28_RS33805_lanthipeptide

Lanthipeptides: BEH84_RS33640_lanthipeptide

1

TDTSSFLTIVCC

12

T+++ FL+I CC

23

TESTPFLSIFCC

34

15.8% NZ_LT969517.1and 1 other:

NZ_QVLV01000012.1 Lanthipeptides: DXC51_RS29565_lanthipeptide

Lanthipeptides: C1733_RS32195_lanthipeptide

1

TDTSSFLTIVCC

12

T ++ FL+I CC

27

TASTGFLSIFCC

38

15.4% NZ_JADNBM010000025.1and 2 others:

NZ_JAQDZW010000013.1 Lanthipeptides: PGT55_RS13345_lanthipeptideNZ_QVMC01000003.1 Lanthipeptides: DWV46_RS15805_lanthipeptide

Lanthipeptides: I2A62_RS14145_lanthipeptide

4

SSFLTIVCC

12

++FLTI CC

31

TAFLTIFCC

39

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg38_10 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 1198.6 Da

Molecular weight: 1199.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1217.4 Da N = 1; 1235.4 Da N = 2; 1253.4 Da N = 3

gnlProkkaGPGAIPCF_75 - Region 1 - ranthipeptide

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Location: 14,685 - 26,245 nt. (total: 11,561 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaGPGAIPCF_122 - Region 1 - RRE-containing

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Location: 1 - 10,463 nt. (total: 10,463 nt) Show pHMM detection rules used

Region on contig edge.

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaGPGAIPCF_126 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 8,836 nt. (total: 8,836 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.