GCA_020562075.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaLJOLFEFE_222 (original name was: gnl|Prokka|LJOLFEFE_222)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	T3PKS	1,961	43,136

gnlProkkaLJOLFEFE_244 (original name was: gnl|Prokka|LJOLFEFE_244)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	hglE-KS	1	25,368

gnlProkkaLJOLFEFE_343 (original name was: gnl|Prokka|LJOLFEFE_343)

Region	Type	From	To	Most similar known cluster		Similarity
Region 21.1	lassopeptide	4,074	25,618

gnlProkkaLJOLFEFE_696 (original name was: gnl|Prokka|LJOLFEFE_696)

Region	Type	From	To	Most similar known cluster		Similarity
Region 49.1	RiPP-like	60,022	78,807

gnlProkkaLJOLFEFE_807 (original name was: gnl|Prokka|LJOLFEFE_807)

Region	Type	From	To	Most similar known cluster		Similarity
Region 50.1	RiPP-like	73,881	81,194	quinolidomicin A	Polyketide	3%

Region	Type	From	To	Most similar known cluster	Similarity
Region 9.1	T3PKS	1,961	43,136
Region 11.1	hglE-KS	1	25,368
Region 21.1	lassopeptide	4,074	25,618
Region 49.1	RiPP-like	60,022	78,807
Region 50.1	RiPP-like	73,881	81,194	quinolidomicin A	Polyketide	3%

Compact view

gnlProkkaLJOLFEFE_222 - Region 1 - T3PKS

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Location: 1,961 - 43,136 nt. (total: 41,176 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaLJOLFEFE_244 - Region 1 - hglE-KS

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Location: 1 - 25,368 nt. (total: 25,368 nt) Show pHMM detection rules used

Region on contig edge.

hglE-KS: (hglE or hglD)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 25368:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mxmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg11_1

PKS_AT (30..322): mxmal

ATSignature: (unknown)

No matches above 50%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 21.7
Methylmalonyl-CoA: 17.9
Malonyl-CoA: 10.8
Propionyl-CoA: 10.6
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

gnlProkkaLJOLFEFE_343 - Region 1 - lassopeptide

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Location: 4,074 - 25,618 nt. (total: 21,545 nt) Show pHMM detection rules used

Region on contig edge.

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

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Gene details

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Gene overview

KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lasso peptide predictions

Lassopeptide(s) for allorf_16589_16747

allorf_16589_16747

Leader:

MLWKNRKETVMYQKPTITKIGNYRTLTN

Full core:

GAGKARERDLLGFSALIVIHWPWG

Putative macrolactam:

GAGKARERD

antiSMASH-DB 4.0 matches for allorf_16589_16747

87.5% NZ_NCVC01000017.1: Lassopeptides: B7703_RS09865_lassopeptide

1

GAGKARERDLLGFSALIVIHWPW

23

GAG ARERDLLGF ALIVIHWPW

1

GAGTARERDLLGFRALIVIHWPW

23

66.7% NZ_FNCN01000047.1: Lassopeptides: BLQ73_RS31810_lassopeptide

3

GKARERDLLGFSALIVIHWP

22

G ARERD+LGF ALIVIH P

3

GSARERDILGFRALIVIHPP

22

50.0% NZ_CP118615.1: Lassopeptides: PVK37_RS09555_lassopeptide

3

GKARERDLLGFSALIVIH

20

G A ERD+ G+ ALIVIH

3

GSADERDIFGYRALIVIH

20

with 2 more sequence matches

29.2% NZ_CP113264.1: Lassopeptides: OUQ99_RS00955_lassopeptide

3

GKARERDLLGFSAL

16

G+ D+LGF AL

3

GRLSCNDILGFRAL

16

20.8% NZ_CP030117.1and 1 other:

NZ_CP042161.1 Lanthipeptides: allorf_1767960_1768070_lanthipeptide

Lanthipeptides: allorf_3078198_3078308_lanthipeptide

18

VIHWPWG

24

+ HWPWG

7

ICHWPWG

13

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lasso peptides

Core peptide predictions

allorf_16589_16747 - Class II: Cleavage pHMM score: 0.10; RODEO score: 21; Monoisotopic mass: 2630.4 Da; Molecular weight: 2632.0 Da; Number of bridges: 0

gnlProkkaLJOLFEFE_696 - Region 1 - RiPP-like

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Location: 60,022 - 78,807 nt. (total: 18,786 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaLJOLFEFE_807 - Region 1 - RiPP-like

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Location: 73,881 - 81,194 nt. (total: 7,314 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1